|
KMID : 0606920090170040403
|
|
Biomolecules & Therapeutics
2009 Volume.17 No. 4 p.403 ~ p.411
|
|
|
Anti-Cancer Effect of the Combination of Thiacremonone and Docetaxel by Inactivation of NF-kB in Human Cancer Cells
|
|
Ban Jung-Ok
Lee Ung-Soo
Noh Jin-Woo
Moon Dong-Cheul
Jeong Heon-Sang
Lee Hee-Soon
Hurh Byung-Serk
Jung Jae-Kyung
Han Sang-Bae
Hong Jin-Tae
Cho Jin-Suk
Hwang In-Guk
Kim Wun-Jae
|
|
|
Abstract
|
|
|
|
Thiacremonone, the main component isolated from heated garlic (Allium sativum L.), is interested for using as a cancer preventive or therapeutic agent since garlic has been known to be useful plant in the treatment of cancers. Nuclear factor kappaB (NF-kB) is constitutively activated in the prostate cancer and activation of NF-kB is implicated in drug resistance in cancer cells. Docetaxel, a semisynthetic analog of paclitaxel, is an antineoplastic drug widely used for advanced various cancer. In previous studies, we found that thiacremonone inhibited activation of NF-kB in cancer cells and marcrophages. In the present study, we investigated whether thiacremonone could increase susceptibility of prostate cancer cells (PC-3 and DU145) to docetaxel via inactivation of NF-kB. We found that the combination treatment of thiacremonone (50 mg/ml) with docetaxel (5 nM) was more effective in the inhibition of prostate cancer cell growth and induction of apoptosis accompanied with the significant inhibition of NF-kB activity than those by the treatment of thiacremonone or docetaxel alone. It was also found that NF-kB target gene expression of Bax, caspase-3 and caspase-9 was much more significantly enhanced, but the expression of Bcl-2 was also much more significantly inhibited by the combination treatment. These results indicate that thiacremonone inhibits NF-kB, and enhances the susceptibility of prostate cancer cells to docetaxel. Thus, thiacremonone could be useful as an adjuvant anti-cancer agent.
|
|
|
KEYWORD
|
|
|
Thiacremonone, Docetaxel, NF-kB, Apoptotic cell death, Prostate cancer
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|